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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial purpose in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are described. Two added splice variants were being claimed but detailed nucleotide sequence details is not really still accessible.
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Blocking peptides are peptides that bind precisely for the target antibody and block antibody binding. These peptide generally is made up of the epitope recognized through the antibody.
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This manual outlines even more feasible optimization of mobile and immunoassay parameters to guarantee the best possible success are...
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This information outlines even here more doable optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
Antibodies bound to the blocking peptide no longer bind towards the epitope within the target protein. By comparing the staining through the blocked antibody vs . the antibody on your own, one can see which staining is precise.
Target Information Cyclin E belongs into the hugely conserved cyclin loved ones, whose associates are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and capabilities like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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